There is increasing demand for innovative locally grown food products. Nationwide, innovation is occurring in research and development, growing, production, distribution and preparation of food. As demonstrated by a nationwide trend in communities around the United States, food innovation has the potential to create economic and community benefits as well as promote food security and public health. This guide provides methods and best practices used by others to create food innovation districts. While this guide does not recommend a specific location for a food innovation district in Hillsborough County, it does provide useful resources and best practices which can be used by local governments, community groups, and individuals.